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Produzione di lisina da parte di Corynebacteruim glutamicum Homoserine (-)

Produzione di lisina da parte di Corynebacteruim glutamicum Homoserine (-)

Hiba Ryma BOUDECHICHA / Imane GUEMRA

89,38 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786203897616
89,38 €
IVA incluido
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Sobre IBD

Lo scopo del nostro lavoro è stato quello di studiare l’effetto dell’acetato di ammonio come fonte di carbonio e azoto sulla produttività dei mutanti omoserini (-) auxotrofi di Corynebacterium glutamicum. A tal fine, abbiamo inoculato quattro terreni contenenti una fonte di carbonio (glucosio; glucosio e acetato di ammonio; acetato di ammonio; acetato di ammonio e acetato di sodio) con un mutante omoserina (-) auxotrofo di C.glutamicum. Durante questa fermentazione, abbiamo monitorato la produzione di biomassa (utilizzando la turbidimetria) e la produzione di lisina da parte del ceppo, misurando al contempo il glucosio e l’acetato di ammonio consumati. Abbiamo scoperto che in un terreno a base di acetato di ammonio, la fermentazione è stata caratterizzata da una notevole produzione di lisina (11,45 g/l) e da una bassa produzione di biomassa.Parole chiave: Corynebacterium glutamicum, auxotrofi, omoserina(-), mutazione, acetato di ammonio, glucosio, lisina, inibizione a feedback.

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