Inicio > Matemáticas y ciencia > Biologia, ciencias de la vida > Produção de lisina por Corynebacteruim glutamicum Homoserina (-)
Produção de lisina por Corynebacteruim glutamicum Homoserina (-)

Produção de lisina por Corynebacteruim glutamicum Homoserina (-)

Hiba Ryma BOUDECHICHA / Imane GUEMRA

89,38 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786203897630
89,38 €
IVA incluido
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O objetivo do nosso trabalho foi estudar o efeito do acetato de amónio como fonte de carbono e de azoto na produtividade de mutantes homoserina (-) auxotróficos de Corynebacterium glutamicum. Para tal, inoculámos quatro meios contendo uma fonte de carbono (glucose; glucose e acetato de amónio; acetato de amónio; acetato de amónio e acetato de sódio) com um mutante de C.glutamicum auxotrófico de homoserina (-). Durante esta fermentação, monitorizámos a produção de biomassa (utilizando turbidimetria) e a produção de lisina pela estirpe, enquanto medimos a glucose e o acetato de amónio consumidos. Verificámos que, num meio à base de acetato de amónio, a fermentação se caracterizou por uma notável produção de lisina (11,45 g/l) e por uma baixa produção de biomassa.Palavras chave: Corynebacterium glutamicum, auxótrofos, homoserina(-), mutação, acetato de amónio, glicose, lisina, inibição por feedback.

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