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Production de lysine par Corynébacteruim glutamicum Homoserine(-)

Production de lysine par Corynébacteruim glutamicum Homoserine(-)

Hiba Ryma BOUDECHICHA / Imane GUEMRA

75,63 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786206734772
75,63 €
IVA incluido
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Sobre IBD

L’objectif de notre travail est d’étudier l’effet de l’acétate d’ammonium comme source de carbone et d’azote sur la productivité des mutants de Corynebacterium glutamicum auxotrophes homoserine (-). Pour se faire, nous avons ensemencé quatre milieux contenant comme source de carbone (glucose ; glucose et acétate d’ammonium ; l’acétate d’ammonium ; l’acétate d’ammonium et acétate de sodium) par un mutant de C.glutamicum auxotrophe homoserine (-). Durant cette fermentation, nous avons suivi la production de biomasse (par la turbidimétrie) ainsi que la production de lysine par la souche, tout en dosant le glucose et l’acétate d’ammonium consommés. Nous avons constaté que dans un milieu à base d’acétate d’ammonium, la fermentation est caractérisée par une production remarquable de la lysine (11,45 g/l) et une faible production de la biomasse.Mots clés : Corynebacterium glutamicum, auxotrophes, homoserine(-), mutation, acétate d’ammonium, glucose, lysine, rétro-inhibition.

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