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Lysinproduktion durch Corynébacteruim glutamicum Homoserin (-)

Lysinproduktion durch Corynébacteruim glutamicum Homoserin (-)

Hiba Ryma BOUDECHICHA / Imane GUEMRA

89,38 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786203897586
89,38 €
IVA incluido
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Sobre IBD

Ziel unserer Arbeit ist es, die Wirkung von Ammoniumacetat als Kohlenstoff- und Stickstoffquelle auf die Produktivität von auxotrophen Homoserin (-)-Mutanten von Corynebacterium glutamicum zu untersuchen. Dazu haben wir vier Medien, die als Kohlenstoffquelle (Glukose; Glukose und Ammoniumacetat; Ammoniumacetat; Ammoniumacetat und Natriumacetat) enthielten, mit einer auxotrophen C.glutamicum-Mutante Homoserine (-) geimpft. Während dieser Fermentation verfolgten wir die Biomasseproduktion (mittels Turbidimetrie) sowie die Lysinproduktion des Stammes und bestimmten gleichzeitig die verbrauchte Glukose und das Ammoniumacetat. Wir stellten fest, dass in einem Medium auf Ammoniumacetatbasis die Fermentation durch eine bemerkenswerte Lysinproduktion (11,45 g/l) und eine geringe Biomasseproduktion gekennzeichnet ist.Schlüsselwörter: Corynebacterium glutamicum, auxotroph, Homoserin(-), Mutation, Ammoniumacetat, Glukose, Lysin, Retro-Inhibition.

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