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Sviluppo e convalida del metodo per la stima del Voriconazolo

Sviluppo e convalida del metodo per la stima del Voriconazolo

K. Vara Prasada Rao / Yarguntla Srinivasa Rao

65,74 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Farmacología
ISBN:
9786209041402
65,74 €
IVA incluido
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Per la determinazione del voriconazolo è stato sviluppato e validato un metodo RP-HPLC rapido, sensibile e specifico con rivelazione UV. La cromatografia è stata eseguita su una colonna Enable C18 G (250 × 4,6 mm) in modalità isocratica, utilizzando una fase mobile di metanolo e acetonitrile (60:40, v/v). La velocità di flusso è stata impostata a 1 mL/min e l’effluente è stato monitorato a 223 nm. L’analisi cromatografica ha rivelato un picco principale per il voriconazolo a un tempo di ritenzione di 2,841 minuti. Il metanolo è stato scelto come solvente per l’analisi e la λmax è stata determinata a 223 nm. Il metodo ha mostrato linearità in un intervallo di concentrazione di 5-30 µg/mL. I parametri di validazione, tra cui linearità, intervallo, precisione, accuratezza, limite di rilevazione (LOD) e limite di quantificazione (LOQ), sono stati valutati e hanno soddisfatto gli standard richiesti.

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Otros libros del autor

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