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Opracowanie i walidacja metody szacowania stężenia worykonazolu

Opracowanie i walidacja metody szacowania stężenia worykonazolu

K. Vara Prasada Rao / Yarguntla Srinivasa Rao

65,74 €
IVA incluido
Disponible
Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Farmacología
ISBN:
9786209043963
65,74 €
IVA incluido
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Opracowano i zwalidowano szybką, czułą i specyficzną metodę RP-HPLC z detekcją UV do oznaczania worykonazolu. Chromatografię przeprowadzono na kolumnie Enable C18 G (250 × 4,6 mm) w trybie izokratycznym, stosując fazę ruchomą metanolu i acetonitrylu (60:40, v/v). Szybkość przepływu ustawiono na 1 ml/min, a ścieki monitorowano przy 223 nm. Analiza chromatograficzna ujawniła główny pik dla worykonazolu przy czasie retencji 2,841 minuty. Jako rozpuszczalnik do analizy wybrano metanol, a λmax określono na 223 nm. Metoda wykazywała liniowość w zakresie stężeń 5-30 µg/ml. Parametry walidacyjne, w tym liniowość, zakres, precyzja, dokładność, granica wykrywalności (LOD) i granica oznaczalności (LOQ), zostały ocenione i spełniły wymagane normy.

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