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Caracterização molecular de Fusarium sp. usando marcadores SCAR

Caracterização molecular de Fusarium sp. usando marcadores SCAR

Debashre Bhattacharjee

76,25 €
IVA incluido
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Botánica y ciencias de las plantas
ISBN:
9786202436809
76,25 €
IVA incluido
Disponible

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Sobre IBD

Este livro trata do desenvolvimento de marcadores SCAR putativos a partir de bandas monomórficas de primers RAPD e ISSR. Os primers utilizados para a identificação de Fusarium oxysporum f.sp.ciceri foram ISSR (GA)8YT ,(ATG)6,(GA)8T) e RAPD, OPA13, OPA18 e OPB14. As raças selecionadas para estudo foram a raça tipo 1 (Hyderabad e Akola), a raça tipo 2 (Kanpur), a raça tipo 3 (Gurdaspur) e a raça tipo 4 (Nova Deli e Jabalpur). Estes iniciadores mostraram bandas monomórficas, que podem ser utilizadas para o desenvolvimento de marcadores SCAR (Sequence Characterized Amplified Regions) para a identificação de Fusarium oxysporum f.sp.ciceri. Os amplicons de 600 pb (ATG)6, 700 pb (GA) 8YT e 650 pb de ISSR e 750 pb (OPA18), 1,4 kb (OPB14), 1500 e 800 pb de OPA 13 foram eluídos, isolados, confirmados e sequenciados. Os iniciadores foram concebidos com a informação sequenciada destes dois fragmentos utilizando o software primer 3 e foram desenvolvidos marcadores SCAR putativos. As raças 1 e 4 apresentaram padrões de virulência próximos em diferenciais de grão-de-bico.

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Otros libros del autor

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