Inicio > Matemáticas y ciencia > Biologia, ciencias de la vida > TA Cloning
TA Cloning

TA Cloning

N. VIJAYALAKSHMI

65,55 €
IVA incluido
Disponible
Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786208450380
65,55 €
IVA incluido
Disponible

Selecciona una librería:

  • Librería Samer Atenea
  • Librería Aciertas (Toledo)
  • Kálamo Books
  • Librería Perelló (Valencia)
  • Librería Elías (Asturias)
  • Donde los libros
  • Librería Kolima (Madrid)
  • Librería Proteo (Málaga)
Sobre IBD

We have exploited the technique of TA cloning of a gene responsible for antibiotic resistance i.e Kanamycin resistance by following the basic steps involved in the cloning process.Background of the invention An essential requirement for effective genetic engineering of bacteria and other cells propagated in cell cultures is the capacity to select the cells with a specific genotypic alteration. The most common selection strategy in recombinant DNA technology is to include a selection marker in the cloning vector or plasmid. A selection marker can be a cloned gene or a DNA sequence, which allows the separation of the host cells containing the selection marker from those not containing it. The selection marker together with a suitable selection medium maintains the cloning vector in the cells. Otherwise, since the replication of plasmids is an energetic burden for the bacterial host, in a growing cultures the bacteria, which have lost the plasmid, would have a growth advantage over the cells with the plasmid.

Artículos relacionados

  • Viral Replication Complexes
    Viruses are obligate intracellular parasites that need to co-opt a living cell’s machinery for replication. At the heart of the viral replication machinery are the nucleic acid polymerases, which are responsible for efficiently copying the viral genome. This process must often be coordinated with other viral processes, including protein translation and viral packaging. The poly...
  • Advances in Flavivirus Research
    The flaviviruses are composed almost entirely of arthropod-borne viruses, a subset of which are responsible for millions of cases of human disease each year. Among these viruses are dengue virus—a scourge throughout the tropical regions of Asia and the Americas; yellow fever virus—the “original” hemorrhagic fever virus; and the recently emerged Zika virus. While the flaviviruse...
    Disponible

    51,85 €

  • Brain Asymmetry of Structure and/or Function
    Lesley J. Rogers / Lesley JRogers
    This edited book brings together research reports on the asymmetry of brain function in various species, including humans, dogs, birds, lizards and bees. As shown in a wide range of species, and, as we now know, not solely in humans, the left and right sides of the brain process information in different ways and control different responses or patterns of behaviour. Since this d...
    Disponible

    51,71 €

  • Fungal Pigments
    With the impact of globalization in research trends, the search for healthier life styles, the increasing public demand for natural, organic, and ‘clean labelled’ products, as well as the growing global market for natural colorants in economically fast-growing countries all over the world, filamentous fungi started to be investigated as readily available sources of chemically d...
    Disponible

    43,80 €

  • The Epithelialto- Mesenchymal Transition ( EMT ) in Cancer
    Joëlle Roche
    The epithelial-to-mesenchymal transition (EMT) is a highly dynamic process with multiple transitional states, by which epithelial cells can convert into a mesenchymal phenotype. This process involves loss of cellular adhesion and cellular polarity, and an improvement in migratory and invasive properties. It occurs during normal embryonic development, tissue regeneration, organ ...
    Disponible

    67,86 €

  • The Biology and Treatment of Myeloid Leukaemias
    There has been an observed decrease in the global mortality from cancer, mostly atributable to improved, particularly early, detection and prevention. For many carcinomas and leukaemias in adults, once the disease has reached a certain stage there are no therapies that are able to erradicate the cancer cells and cure patients. There has been progress in the treatment of acute m...
    Disponible

    51,85 €

Otros libros del autor

  • Klonowanie TA
    N. VIJAYALAKSHMI
    Wykorzystaliśmy technikę klonowania TA genu odpowiedzialnego za oporność na antybiotyki, tj. oporność na kanamycynę, wykonując podstawowe kroki związane z procesem klonowania. Tło wynalazku Podstawowym wymogiem skutecznej inżynierii genetycznej bakterii i innych komórek rozmnażanych w kulturach komórkowych jest zdolność do selekcji komórek z określoną zmianą genotypową. Najczęs...
    Disponible

    65,49 €

  • TA-Klonen
    N. VIJAYALAKSHMI
    Wir haben die Technik des TA-Klonens eines Gens, das für die Antibiotikaresistenz, d.h. die Kanamycin-Resistenz, verantwortlich ist, genutzt, indem wir die grundlegenden Schritte des Klonierungsprozesses nachvollzogen haben.Hintergrund der Erfindung Eine wesentliche Voraussetzung für eine wirksame gentechnische Veränderung von Bakterien und anderen Zellen, die in Zellkulturen v...
    Disponible

    65,49 €

  • Clonagem TA
    N. VIJAYALAKSHMI
    Explorámos a técnica de clonagem TA de um gene responsável pela resistência a antibióticos, ou seja, a resistência à canamicina, seguindo os passos básicos envolvidos no processo de clonagem.Contexto da invenção Um requisito essencial para a engenharia genética eficaz de bactérias e outras células propagadas em culturas celulares é a capacidade de selecionar as células com uma ...
    Disponible

    65,49 €

  • Clonage TA
    N. VIJAYALAKSHMI
    Nous avons exploité la technique de clonage TA d’un gène responsable de la résistance aux antibiotiques, à savoir la résistance à la kanamycine, en suivant les étapes de base du processus de clonage. Contexte de l’invention Une condition essentielle pour un génie génétique efficace des bactéries et autres cellules propagées dans des cultures cellulaires est la capacité de sélec...
    Disponible

    65,49 €

  • Clonazione TA
    N. VIJAYALAKSHMI
    Abbiamo sfruttato la tecnica del clonaggio TA di un gene responsabile della resistenza agli antibiotici, ossia la resistenza alla kanamicina, seguendo le fasi fondamentali del processo di clonazione.Contesto dell’invenzione Un requisito essenziale per un’efficace ingegneria genetica dei batteri e di altre cellule propagate in colture cellulari è la capacità di selezionare le ce...
    Disponible

    65,49 €

  • USO DELLA RT-PCR IN TEMPO REALE
    N. VIJAYALAKSHMI
    Il virus dell’afta epizootica (FMDV) viene diagnosticato mediante test di isolamento del virus combinato con l’antigene ELISA e, recentemente, con la RT-PCR in un unico passaggio. L’uso della RT-PCR fluorogenica sta assumendo un’importanza sempre maggiore per la rilevazione rapida dell’FMDV da campioni clinici che non possono essere trattati con i normali test di isolamento del...
    Disponible

    64,24 €