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Klonowanie TA

Klonowanie TA

N. VIJAYALAKSHMI

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786208873929
65,49 €
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Wykorzystaliśmy technikę klonowania TA genu odpowiedzialnego za oporność na antybiotyki, tj. oporność na kanamycynę, wykonując podstawowe kroki związane z procesem klonowania. Tło wynalazku Podstawowym wymogiem skutecznej inżynierii genetycznej bakterii i innych komórek rozmnażanych w kulturach komórkowych jest zdolność do selekcji komórek z określoną zmianą genotypową. Najczęstszą strategią selekcji w technologii rekombinacji DNA jest włączenie markera selekcyjnego do wektora klonującego lub plazmidu. Markerem selekcyjnym może być sklonowany gen lub sekwencja DNA, która umożliwia oddzielenie komórek gospodarza zawierających marker selekcyjny od tych, które go nie zawierają. Marker selekcyjny wraz z odpowiednią pożywką selekcyjną utrzymuje wektor klonujący w komórkach. W przeciwnym razie, ponieważ replikacja plazmidów jest obciążeniem energetycznym dla gospodarza bakteryjnego, w rosnących kulturach bakterie, które utraciły plazmid, miałyby przewagę wzrostu nad komórkami z plazmidem.

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