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Sequência de inserção IS6110 de Mycobacterium tuberculosis por PCR-RFLP

Sequência de inserção IS6110 de Mycobacterium tuberculosis por PCR-RFLP

Irum Naheed Azra Khanum

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786208745912
49,74 €
IVA incluido
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Sobre IBD

A tuberculose (TB) é a principal causa de morte no mundo causada pelo Mycobacterium tuberculosis (M. tb). Segundo a OMS, a TB mata 5000 pessoas por dia e aproximadamente três milhões de pessoas por ano. A taxa de prevalência da TB tem vindo a aumentar de dia para dia no Paquistão. O aparecimento de estirpes MDR de M. tb é motivo de grande preocupação epidemiológica. A resistência do M. tb aos antibióticos deve-se a mutações em determinados genes. Neste estudo, as mutações associadas à resistência ao etambutol (EMB) foram determinadas através da análise RFLP. Foram pesquisadas alterações nas posições de códão 306 e 497 do gene embB. O IS6110 é um elemento de inserção que se encontra exclusivamente nos membros do complexo M. tuberculosis (MTBC) e actua como uma importante ferramenta de diagnóstico na identificação destas espécies. O ADN foi extraído de uma cultura de M. tb e submetido a digestão pela enzima de restrição PvuII. Em seguida, o padrão de RFLPs foi estudado por meio de Southern blotting para verificar a diversidade. O número de cópias de IS6110 varia nestes isolados. Os isolados de M. tb de áreas estreitamente relacionadas apresentam mais semelhanças do que os isolados de áreas distantes.

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