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Analiza molekularna ryzobakterii diazotroficznych w ryżu

Analiza molekularna ryzobakterii diazotroficznych w ryżu

Mukund Chandra Thakur

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2026
Materia
Ciencia: cuestiones generales
ISBN:
9786630024524
101,20 €
IVA incluido
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Sobre IBD

Dwie tradycyjne odmiany ryżu, Sataria i Kartki, zostały zebrane z określonych pól położonych w dystrykcie Madhubani w północnym Biharze. Szeroki zakres bakterii wiążących azot (diazotrofów) został wyizolowany z korzeni tych dwóch odmian ryżu przy użyciu wzbogacania w bezazotowe pożywki półstałe;Używając sond oligonukleotydowych ukierunkowanych na rybosomalny RNA, 53 izolaty z odmiany Sataria i 137 izolatów z odmiany ryżu Kartki zidentyfikowano jako eubakterie. Spośród tych 190 izolatów, większość z nich (183) zidentyfikowano jako α-, β- lub γ- proteobakterie. Wykorzystując specyficzne dla grupy sondy oligonukleotydowe, 14 izolatów przypisano do α-, 137 do β- i 32 do γ-podgrupy proteobakterii. Pięć różnych endofitycznych ryzobakterii diazotroficznych (Herbaspirillum seropedica, Gluconobacter diazotrophicus, Azospirillum brasilense, Burkholderia cepacia i Pseudomonas sp.) inokulowano aksenicznie uprawianymi sadzonkami ryżu Kartki i analizowano ich wpływ na wzrost i plon. Spośród wszystkich pięciu bakterii diazotroficznych, B. cepacia wykazała najwyższy wzrost roślin i plonu ziarna.

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