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Analisi molecolare dei rizobatteri diazotrofi nel riso

Analisi molecolare dei rizobatteri diazotrofi nel riso

Mukund Chandra Thakur

101,20 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2026
Materia
Ciencia: cuestiones generales
ISBN:
9786630088519
101,20 €
IVA incluido
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Sobre IBD

Due varietà di riso tradizionali, Sataria e Kartki, sono state raccolte da campi specifici situati nel distretto di Madhubani, nel Bihar settentrionale. Un’ampia gamma di batteri azotofissatori (diazotrofi) è stata isolata dalle radici di queste due varietà di riso mediante arricchimento in terreni semisolidi privi di azoto;Utilizzando sonde oligonucleotidiche dirette all’RNA ribosomiale, 53 isolati da Sataria e 137 isolati dalla varietà di riso Kartki sono stati identificati come eubatteri. Di questi 190 isolati, la maggior parte (183) sono stati identificati come proteobatteri α-, β- o γ-. Utilizzando sonde oligonucleotidiche specifiche per il gruppo, 14 isolati sono stati assegnati all’α-, 137 al β- e 32 al sottogruppo γ dei proteobatteri. Cinque diversi rizobatteri endofiti diazotrofi (Herbaspirillum seropedica, Gluconobacter diazotrophicus, Azospirillum brasilense, Burkholderia cepacia e Pseudomonas sp.) sono stati inoculati su piantine di riso Kartki cresciute per via assenica e ne è stato analizzato l’impatto sulla crescita e sulla resa. Tra i cinque batteri diazotrofi, B. cepacia ha mostrato il più alto incremento nella crescita delle piante e nella resa in grani.

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