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Un transgène bactérien pour la catalase protège la traduction de la protéine D1

Un transgène bactérien pour la catalase protège la traduction de la protéine D1

Khaled AlTaweel

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786207819218
73,58 €
IVA incluido
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Sobre IBD

Résumé Le vecteur binaire pBI101-katE contenant le gène katE dérivé d’Escherichia coli et régulé par le promoteur du gène de la petite sous-unité de la Rubisco de la tomate, rbcS3C, a été isolé et caractérisé moléculairement, puis transformé en cellules compétentes à l’aide de la méthode du choc thermique. Le vecteur binaire a été transformé en cellules compétentes d’Agrobacterium tumefaciens de deux manières : la première par la méthode de croisement tri-parental, et la seconde par la méthode d’électroporation. La construction pBI101-katE a été introduite dans une tomate naine par transformation médiée par Agrobacterium tumefaciens. La construction a également été introduite dans un tabac par bombardement biolistique. Des plantes transgéniques putatives ont été régénérées et sélectionnées à l’aide de la PCR. L’analyse PCR des plantes T1 issues de callosités indépendantes a révélé la présence des gènes katE et nptII.

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Otros libros del autor

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    Abstract Il vettore binario pBI101-katE contenente il gene katE derivato dall’Escherichia coli e guidato dal promotore del gene della subunità piccola della Rubisco del pomodoro, rbcS3C, è stato isolato e caratterizzato molecolarmente, quindi trasformato in cellule competenti utilizzando il metodo dello shock termico. Il vettore binario è stato trasformato in cellule competenti...
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    Resumo O vetor binário pBI101-katE, que contém o gene katE derivado da Escherichia coli e impulsionado pelo promotor do gene da subunidade pequena da Rubisco do tomate, rbcS3C, foi isolado e caracterizado molecularmente, sendo depois transformado em células competentes utilizando o método de choque térmico. O vetor binário foi transformado em células competentes de Agrobacteriu...
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