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Um transgénico bacteriano para a catalase protege a tradução da proteína D1

Um transgénico bacteriano para a catalase protege a tradução da proteína D1

Khaled AlTaweel

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786207819249
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Resumo O vetor binário pBI101-katE, que contém o gene katE derivado da Escherichia coli e impulsionado pelo promotor do gene da subunidade pequena da Rubisco do tomate, rbcS3C, foi isolado e caracterizado molecularmente, sendo depois transformado em células competentes utilizando o método de choque térmico. O vetor binário foi transformado em células competentes de Agrobacterium tumefaciens de duas maneiras: a primeira através do método de acasalamento triparental e a segunda pelo método de eletroporação. A construção pBI101-katE foi introduzida num tomate anão através da transformação mediada por Agrobacterium tumefaciens. Além disso, a construção foi introduzida num tabaco por bombardeamento biolístico. Plantas transgénicas putativas foram regeneradas e posteriormente selecionadas por PCR. A análise por PCR de plantas T1 a partir de calos independentes revelou a presença dos genes katE e nptII.

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Otros libros del autor

  • Un transgene batterico per la catalasi protegge la traduzione della proteina D1
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    Abstract Il vettore binario pBI101-katE contenente il gene katE derivato dall’Escherichia coli e guidato dal promotore del gene della subunità piccola della Rubisco del pomodoro, rbcS3C, è stato isolato e caratterizzato molecolarmente, quindi trasformato in cellule competenti utilizzando il metodo dello shock termico. Il vettore binario è stato trasformato in cellule competenti...
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    Zusammenfassung Der binäre Vektor pBI101-katE, der das aus Escherichia coli stammende katE-Gen enthält und vom Promotor des Rubisco-Kleinsubunit-Gens der Tomate, rbcS3C, gesteuert wird, wurde isoliert, molekular charakterisiert und anschließend mittels der Hitzechockmethode in kompetente Zellen transformiert. Der binäre Vektor wurde auf zwei Arten in kompetente Agrobacterium tu...
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    Résumé Le vecteur binaire pBI101-katE contenant le gène katE dérivé d’Escherichia coli et régulé par le promoteur du gène de la petite sous-unité de la Rubisco de la tomate, rbcS3C, a été isolé et caractérisé moléculairement, puis transformé en cellules compétentes à l’aide de la méthode du choc thermique. Le vecteur binaire a été transformé en cellules compétentes d’Agrobacter...
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    Streszczenie Wektor binarny pBI101-katE zawierający gen katE pochodzący z Escherichia coli i sterowany promotorem genu małej podjednostki Rubisco pomidora, rbcS3C, został wyizolowany i scharakteryzowany molekularnie, a następnie transformowany do komórek kompetentnych metodą szoku cieplnego. Wektor binarny został transformowany do kompetentnych komórek Agrobacterium tumefaciens...
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