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Studi tassonomici su Phlebotomus (Larroussius) orientalis del Sudan

Studi tassonomici su Phlebotomus (Larroussius) orientalis del Sudan

Amel Bari / Mo’awia Hassan

83,23 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786209373091
83,23 €
IVA incluido
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Questa ricerca è stata condotta per studiare le differenze morfometriche e molecolari nelle popolazioni di Phlebotomus orientalis provenienti da tre diverse regioni del Sudan. Sono stati raccolti campioni di flebotomi Phlebotomine dagli Stati di Gedaref, White Nile e Khartoum. Gli esemplari di P. orientalis sono stati raccolti utilizzando trappole luminose miniaturizzate CDC e trappole adesive in carta. Gli esemplari di flebotomi sono stati quindi montati su vetrini con terreno di Brelese e identificati a livello di specie. I campioni montati di P. orientalis sono stati poi sottoposti a misurazioni morfometriche di 23 e 29 caratteri rispettivamente per le femmine e i maschi. Inoltre, i geni Cyt b, ITS2 rDNA 18S di P. orientalis provenienti da diverse regioni sono stati amplificati mediante PCR convenzionale e in tempo reale. I campioni amplificati dei geni Cyt b e ITS2 sono stati purificati e sequenziati. Le sequenze sono state assemblate, modificate, analizzate e poi confrontate con il database del GenBank. Le popolazioni di P. orientalis provenienti da tre aree geografiche hanno mostrato un certo grado di differenza sia in base all’analisi morfometrica che a quella molecolare.

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