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Estudos taxonómicos sobre Phlebotomus (Larroussius) orientalis do Sudão

Estudos taxonómicos sobre Phlebotomus (Larroussius) orientalis do Sudão

Amel Bari / Mo’awia Hassan

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786209378218
83,23 €
IVA incluido
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Esta investigação foi realizada para estudar as diferenças morfométricas e moleculares nas populações de Phlebotomus orientalis de três regiões diferentes do Sudão. Amostras de flebotomíneos foram coletadas nos estados de Gedaref, Nilo Branco e Cartum. Os espécimes de P. orientalis foram coletados usando armadilhas luminosas miniatura CDC e armadilhas de papel adesivo. Os espécimes de flebotomíneos foram então montados em lâminas de vidro em meio Brelese e identificados ao nível da espécie. Em seguida, as amostras montadas de P. orientalis foram processadas para medições morfométricas de 23 e 29 caracteres para fêmeas e machos, respectivamente. Além disso, os genes Cyt b, ITS2 rDNA 18S de P. orientalis de diferentes regiões foram amplificados por PCR convencional e em tempo real. As amostras amplificadas dos genes Cyt b e ITS2 foram purificadas e sequenciadas. As sequências foram montadas, editadas, analisadas e, em seguida, comparadas com a base de dados do GenBank. As populações de P. orientalis de três áreas geográficas mostraram algum grau de diferença com base na análise morfométrica e molecular.

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    Badania te przeprowadzono w celu zbadania różnic morfometrycznych i molekularnych w populacjach Phlebotomus orientalis z trzech różnych regionów Sudanu. Próbki muchówek z rodzaju Phlebotomine zostały zebrane w stanach Gedaref, White Nile i Chartum. Okazy P. orientalis zostały zebrane przy użyciu miniaturowych pułapek świetlnych CDC i pułapek z lepkim papierem. Okazy muchówek zo...
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