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Studi sulla produzione di poligalatturonasi da BacillusC4 isolato

Studi sulla produzione di poligalatturonasi da BacillusC4 isolato

M.P. Kusuma

93,65 €
IVA incluido
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Empresa y gestión
ISBN:
9786209363436
93,65 €
IVA incluido
Disponible

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La presente indagine ha lo scopo di studiare la produzione di poligalatturonasi alcalina e termostabile utilizzando Bacillus subtilis (C4) isolato dal suolo mediante fermentazione sommersa. Da sei siti di campionamento sono state isolate in totale 66 colonie batteriche. Delle 66 isolati, 8 hanno mostrato attività poligalatturonasi e l’isolato C4 ha mostrato la massima attività. Il potenziale poligalatturonasi del nuovo isolato è stato testato su tre microrganismi produttori di poligalatturonasi. Comparativamente, l’isolato C4 ha mostrato la massima attività enzimatica ed è stato quindi scelto per uno studio approfondito. L’isolato C4 è stato caratterizzato e identificato come Bacillus subtilis C4. Il nuovo Bacillus subtilis è stato utilizzato per la produzione di poligalatturonasi mediante fermentazione sommersa. È stato studiato il profilo di crescita dell’isolato sopra citato. I risultati hanno rivelato che l’organismo mostrava una fase log massima fino a 24 ore, dopodiché la crescita si è gradualmente ridotta ed è rimasta stabile fino a 72 ore, indicando una fase stazionaria a 400C e pH 11,0. Sono stati studiati parametri di processo quali tempo di incubazione (19,65 U/mL), temperatura (28,08 U/mL), pH 7,0, volume dell’inoculo (1 mL), età dell’inoculo (24 ore) e agitazione (150 giri/min) per ottenere la massima produzione di poligalatturonasi.

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