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1. Guide to the Book Plant Kinases Nico Dissmeyer and Arp Schnittger 2. The Age of Protein Kinases Nico Dissmeyer and Arp Schnittger 3. Expression and Purification of Active Protein Kinases from Wheat Germ Extracts Boglárka Sonkoly, Viola Bardóczy, and Tamás Mészáros 4. Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System Hirofumi Harashima and Masami Sekine 5. Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants Róbert Dóczi, Elizabeth Hatzimasoura, and László Bögre 6. Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks Nico Dissmeyer and Arp Schnittger 7. Bacterial Assay to Study Plant Sensor Histidine Kinases Lukáš Spíchal 8. Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase Julija Umbrasaite, Alois Schweighofer and Irute Meskiene 9. Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen Shutian Li and Vernonica E. Franklin-Tong 10. Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules Dor Salomon, Chao Zhang, Kevan M. Shokat, and Guido Sessa 11. Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser 12. Quantifying Degradation Rates of Trans-Membrane Receptor Kinases Niko Geldner 13. Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta Mark A.C.J. Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J.W.G. Visser, and Sacco C. de Vries 14. Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo Stefan Pusch, Nico Dissmeyer, and Arp Schnittger 15. Chemical Genetic Analysis of Protein Kinase Function in Plants Mike Böhmer, Michael Bölker, and Tina Romeis 16. Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes Thomas Colby, Horst Röhrig, Anne Harzen, and Jürgen Schmidt 17. Phosphoproteomics Using iTRAQ Alexandra M.E. Jones and Thomas S. Nühse
