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Molekularne wykrywanie leiszmanii u komarów z rodzaju Phlebotomus

Molekularne wykrywanie leiszmanii u komarów z rodzaju Phlebotomus

Ilhem Mihoubi / Khalida Frahtia / Stéphane Picot

114,50 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2026
Materia
Biologia, ciencias de la vida
ISBN:
9786209856082
114,50 €
IVA incluido
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Komary z rodzaju Phlebotomus odgrywają istotną rolę w chorobach ludzkich, takich jak leiszmanioza. Walka z wektorami, rozpoczęta przez algierskie służby sanitarne, okazuje się skuteczna, ponieważ leiszmanioza wydaje się ustępować w Algierii. W tym kontekście niniejsze badanie ma na celu molekularne wykrywanie Leishmania u wektora. Ponadto diagnostyka molekularna objęła również próbki skóry pobrane od pacjentów z regionu Constantine. Jeśli chodzi o wektory, schwytano 5858 flebotomów, w tym 4360 samców i 1498 samic, podzielonych na 5 gatunków. W przypadku próbek skórnych 56,96% zebranych próbek dało wynik pozytywny w RT-PCR. Jeśli chodzi o wykrywanie Leishmania u flebotomów za pomocą PCR, wyniki pokazują, że 3 z 60 przeprowadzonych testów PCR rodzaju dały wynik pozytywny. Świadczy to o tym, że wektory były zarażone, jednak identyfikacja gatunku za pomocą RT-PCR nie przyniosła żadnych wyników. Można to wyjaśnić niewystarczającą ilością DNA leiszmanii obecnej u wektora, co w konsekwencji potwierdza hipotezę o regresji leiszmaniozy w Constantine.

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