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This research was observed that HEV-RNA was better than anti-HEV for diagnosis of acute hepatitis but it can not be a better test in routine, as RT-PCR is cumbersome and costly. However, in a setting of acute hepatitis if routine viral markers are negative, HEV RT-PCR may be the next useful tool of investigation.It was also reported that viremia lasts beyond normalization of transaminases in some patients and this may suggest that liver injury is independent from viral replication. Our data also show that HEV isolates in human and sewage in Rajasthan are genetically closely related to each other and belonged to genotype IA. These finding suggest that sewage act as reservoir of HEV for sporadic hepatitis spread. These data may play a valuable role in understanding pathogenesis and transmission of hepatitis E virus.
