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Development of Improved Strains and Optimization of Fermentation Processes Lei Han and Sarad R. Parekh Experimental Design in Microbiology Guillaume E. Vanot and Michelle Sergent Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acid Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, and José-Luis Barredo Production of Erythromycin With Saccharopolyspora erythraea Wolfgang Minas The Phenylacetyl-CoA Catabolon: Genetic, Biochemical, and Biotechnological Approaches José M. Luengo, Belén García, Ángel Sandoval, Elsa Arias-Barrau, Sagrario Arias, Francisco Bermejo, and Elías R. Olivera Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compounds Carmen Méndez and José A. Salas Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerus Paloma Liras and Juan F. Martín Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications Maria Margarida Diogo, João António Queiroz, and Duarte Miguel F. Prazeres Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicum Mutant Masato Ikeda, Junko Ohnishi, and Satoshi Mitsuhashi Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicum Christoph Wittmann and Elmar Heinzle Protein and Vitamin Production in Bacillus megaterium Heiko Barg, Marco Malten, Martina Jahn, and Dieter Jahn Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungi Heidi Sisniega, José-Luis del Río, María-José Amaya, and Ignacio Faus Strain and Culture Conditions Improvement for b-Carotene Production With Mucor Enrique A. Iturriaga, Tamás Papp, Jesper Breum,José Arnau, and Arturo P. Eslava Xanthophylls in Fungi: Metabolic Engineering of the Astaxanthin Biosynthetic Pathway in Xanthophyllomyces dendrorhous Hans Visser, Gerhard Sandmann, and Jan C. Verdoes Methodologies for the Analysis of Fungal Carotenoids Paul D. Fraser and Peter M. Bramley Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypii María A. Santos, Laura Mateos, Karl-Peter Stahmann, and José-Luis Revuelta Improved Polysaccharide Production Using Strain Improvement Thomas P. West Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hosts Stefania Cresti, Cesira L. Galeotti, Serena Schippa, Gian Maria Rossolini, and Maria C. Thaller Gene Expression Arrays in Food Bart Weimer, Yi Xie, Lan-szu Chou, and Adele Cutler Optimization of Proteome Analysis for Wine Yeast Strains Tammi L. Olineka, Apostolos Spiropoulos, Paula A. Mara, and Linda F. Bisson Bacteriocin-Producing Strains in a Meat Environment Frédéric Leroy and Luc De Vuyst In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells Eduardo J. Schiffrin, Nabila Ibnou-Zekri, Jean M. Ovigne, Thierry von der Weid, and Stephanie Blum Volatile Sulfur Detection in Fermented Foods Bart Weimer and Ben Dias Bioleaching: Analysis of Microbial Communities Dissolving Metal Sulfides Axel Schippers and Klaus Bosecker Use of PhoN Phosphatase to Remediate Heavy Metals Marion Paterson-Beedle and Lynne E. Macaskie Safe Dispatch and Transport of Biological Material Vera Weihs and Christine Rohde How to Deposit Biological Material for Patent Purposes Vera Weihs Laws and Regulations for the Protection of Biotechnological Inventions Uwe Fitzner Index