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TABLE OF CONTENTS VOLUME 2 HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS Sylvie Doublié, Editor CHAPTER TITLE AUTHOR(S) PAGE 1. Cryocrystallography of macromolecules: practice and optimisation Elspeth Garman and Robin L. Owen 1-24 2. Determination of reaction intermediates structures in heme proteins Kelvin Chu 1-19 3. Annealing Macromolecular Crystals Gerard J. Bunick and B. Leif Hanson 1-16 4. First Analysis of Macromolecular Crystals: Biochemistry and X-ray Diffraction David Jeruzalmi 1-32 5. X-ray Data-Collection from Macromolecular Crystals Elspeth Garman and Robert M. Sweet 1-44 6. Characterizing your Crystal from an Initial Native Data Set Michael R. Sawaya 1-37 7. Molecular replacement Eric Toth 1-37 8. Phase determination using halide ions Miroslawa Dauter and Zbigniew Dauter 1-11 9. Multiple isomorphous replacement Mark Rould 1-25 10. Substructure determination in MAD, SAD, and SIRAS data using Shake-and-Bake (SnB) P. Lynne Howell, G. David Smith, and Christopher T. Lemke 1-22 11. Substructure determination in isomorphous replacement and anomalous diffraction experiments Ralph Grosse-Kunstleve and Thomas Schneider 1-22 12. Automated structure solution with autoSHARP Clemens Vonrhein, Eric Blanc, Pietro Roversi and Gérard Bricogne 1-24 13. Introduction to Macromolecular Refinement Dale E. Tronrud 1-32 14. Quality control and validation Gerard J. Kleywegt 1-36 15. Crystallographic software: A sustainable resource for the community Stephen Everse and Sylvie Doublié 1-8
