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TABLE OF CONTENTS VOLUME 1 HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS Sylvie Doublié, Editor CHAPTER TITLE AUTHOR(S) PAGE 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh 1-29 2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh 1-26 3. Baculoviral expression of an integral membrane protein for structural studies Dean R. Madden and Markus Safferling 1-33 4. Protein Engineering Sonia Longhi, François Ferron, and Marie-Pierre Egloff 1-48 5. Selenomethionyl protein expression Sylvie Doublié 1-21 6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule Gloria Borgstahl 1-34 7. Screening and optimization methods for non-automated crystallization laboratories Terese Bergfors 1-32 8. Improving Marginal Crystals Charles W. Carter, Jr. and Madeleine Riès-Kautt 1-34 9. Optimisation Techniques for Automation and High Throughput Naomi E. Chayen 1-26 10. Three-dimensional crystallization of membrane proteins James Féthière 1-40 11. Crystallization of Protein-DNA complexes Thomas Hollis 1-20 12. Preparation and crystallization of RNA Barbara Golden 1-24 13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai 1-27
