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L’analisi di fusione multipla ad alta risoluzione come strumento diagnostico

L’analisi di fusione multipla ad alta risoluzione come strumento diagnostico

Kais Kassim Ghaima / Shahad Tariq Hamad

96,45 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2024
Materia
Genética (no médica)
ISBN:
9786208384791
96,45 €
IVA incluido
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Sobre IBD

Lo scopo di questo studio è stato quello di sviluppare una RT-PCR multiplex con analisi di fusione ad alta risoluzione (HRM) per la rilevazione rapida e accurata di cinque carbapenemasi che mantengono il gene 16S rRNA. Sono state progettate sei coppie di primer e le condizioni di RT-PCR secondo il software Beacon designer. L’identificazione dei geni è stata condotta mediante curve di fusione e temperature con Multiplex RT-PCR utilizzando un reagente HRM dedicato. Questo è il primo test che individua i principali geni delle carbapenemasi in un test a due provette, consentendo la rilevazione simultanea di 5 dei più importanti marcatori di resistenza alle cefalosporine di terza generazione e ai carbapenemi.

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