DNA Recombination

Part I. Genetic and Molecular Biological Approaches with Yeast 1. Methods to Study Mitotic Homologous Recombination and Genome Stability Xiuzhong Zheng, Anastasiya Epstein, and Hannah L. Klein 2. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends Wenjian Ma, Jim Westmoreland, Wataru Nakai, Anna Malkova, and Michael A. Resnick 3. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis Sarah Farmer, Wing-Kit Leung, and Hideo Tsubouchi 4. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA Hannah G. Blitzblau and Andreas Hochwagen 5. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes Edgar Hartsuiker 6. Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast Scott Houghtaling, Toyoko Tsukuda, and Mary Ann Osley 7. Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis Jasvinder A. Singh and G. Valentin Börner 8. Mapping of Crossover Sites Using DNA Microarrays Stacy Y. Chen and Jennifer C. Fung 9. Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1 Hsiao-Chi Lo and Nancy M. Hollingsworth 10. Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae Belén Gómez-González, José F. Ruiz, and Andrés Aguilera 11. In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae Samantha Stuckey, Kuntal Mukherjee, and Francesca Storici 12. Detection of RNA-Templated Double-Strand Break Repair in Yeast Ying Shen and Francesca Storici Part II. Genetic and Molecular Biological Approaches with Higher Eukaryotes 13. SNP-Based Mapping of Crossover Recombination in C. elegans Grace C. Bazan and Kenneth J. Hillers 14. Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana Jan Drouaud and Christine Mézard 15. Isolation of Meiotic Recombinants from Mouse Sperm Francesca Cole and Maria Jasin 16. Homologous Recombination Assay for Interstrand Crosslink Repair Koji Nakanishi, Francesca Cavallo, Erika Brunet, and Maria Jasin 17. Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40 Hiroyuki Kitao, Seiki Hirano, and Minoru Takata 18. Understanding the Immunoglobulin Locus Specificity of Hypermutation Vera Batrak, Artem Blagodatski, and Jean-Marie Buerstedde Part III. In Vitro Reconstitution of Homologous Recombination Reactions and Single Molecular Analysis of Recombination Proteins 19. Quality Control of Purified Proteins Involved in Homologous Recombination Xiao-Ping Zhang and Wolf-Dietrich Heyer 20. Assays for Structure-Selective DNA Endonucleases William D. Wright, Kirk T. Ehmsen, and Wolf-Dietrich Heyer 21. In vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis Jie Liu, Jessica Sneeden, and Wolf-Dietrich Heyer 22. An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase Yasuto Murayama and Hiroshi Iwasaki 23. Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro Matth