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Przygotowanie, charakterystyka i ocena in vitro trójwymiarowych rusztowań

Przygotowanie, charakterystyka i ocena in vitro trójwymiarowych rusztowań

Gabriela S. Andrade / Luis E. Almeida / Paulo A. L. Lima

60,32 €
IVA incluido
Disponible
Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Tecnología: cuestiones generales
ISBN:
9786209356292
60,32 €
IVA incluido
Disponible

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Sobre IBD

Scaffolds-3D z chitozanu (CHI), fibroiny (SF) i hydroksyapatytu (HA) są biokompatybilne i atrakcyjne do zastosowań w inżynierii tkankowej kości. W związku z tym celem badania było przygotowanie, scharakteryzowanie i ocena zachowania in vitro scaffolds-3D z CHI, CHI/SF, CHI/SF/HA. Biomateriały zostały wyprodukowane z roztworu CHI, CHI/SF i CHI/SF/HA w kwasie octowym, a po zakończeniu procesów produkcyjnych zostały poddane testom in vitro z komórkami SaOS-2 przez 7, 14 i 21 dni. Scaffoldy scharakteryzowano za pomocą dyfrakcji rentgenowskiej, spektroskopii w podczerwieni z transformacją Fouriera z całkowitą atenuowaną reflektancją, analizy termograwimetrycznej, różnicowej kalorymetrii eksploracyjnej, skaningowej mikroskopii elektronowej, spektroskopii energii dyspersyjnej oraz poddano testom w celu sprawdzenia porowatości, wielkości porów i kąta zwilżania. Żywotność i aktywność komórek oceniono na podstawie redukcji MTT i wykrycia aktywności fosfatazy alkalicznej. Szkielety 3D uznano za nietoksyczne i zdolne do promowania adhezji, proliferacji i różnicowania komórek, dzięki czemu mogą być stosowane w terapii naprawy ubytków kostnych.

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Otros libros del autor

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    3D scaffolds made of chitosan (CHI), fibroin (SF) and hydroxyapatite (HA) are biocompatible and attractive for applications in bone tissue engineering. Therefore, the objective was to prepare, characterise and evaluate the in vitro behaviour of 3D scaffolds made of CHI, CHI/SF and CHI/SF/HA. The biomaterials were produced from a solution of CHI, CHI/SF and CHI/SF/HA in acetic a...
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