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Propagação microclonal de plantas

Propagação microclonal de plantas

Dildora Abduganieva / Guljakhon Eshbekova

93,38 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786209372216
93,38 €
IVA incluido
Disponible

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Este manual metodológico fornece um guia abrangente e orientado para a prática da propagação microclonal de plantas, concebido especificamente para estudantes universitários envolvidos em biotecnologia vegetal baseada em laboratório. Introduz os princípios fundamentais da cultura de tecidos vegetais, incluindo a totipotência celular, o papel dos reguladores de crescimento das plantas e as vias de desenvolvimento da formação de calos, organogénese e embriogénese somática. O manual descreve a organização essencial do laboratório, as normas de segurança, as funções do equipamento e os procedimentos de esterilização necessários para manter as condições assépticas. Uma secção detalhada sobre a preparação dos meios de cultura abrange a composição dos nutrientes, a formulação da solução de reserva, o ajuste do pH e a autoclavagem, complementada por exercícios passo a passo para os alunos. Os capítulos seguintes focam a seleção de plantas dadoras, tipos de explantes, técnicas de esterilização de superfícies e o estabelecimento de culturas. Exercícios práticos guiam os estudantes através de etapas críticas da micropropagação, incluindo inoculação, multiplicação de rebentos, enraizamento e aclimatação de plântulas regeneradas.

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