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Part I Cell Differentiation and Development In Vitro 1 Developmental Biology of Somatic EmbryogenesisR.J. ROSE, F.R. MANTIRI, S. KURDYUKOV, S.-K. CHEN, X.-D. WANG, K.E. NOLAN, and M.B. SHEAHAN Introduction Basic Requirements for In Vitro Somatic Embryogenesis Explant and Stem Cell Biology Earliest Event in Embryogenesis - Asymmetric Cell Division Stress Component in the Initiation of Somatic Embryogenesis Hormones and the Initiation of Somatic Embryogenesis Induction of SE by Over-Expression of Leafy Cotyledon Transcription Factors and their Relationship to SE induction and Repression - the GA Connection ABA, Stress and GA Soluble Signals and Cell -Cell Interactions that Promote SE in Suspension Cultures Development Program after SE induction Concluding Remarks and a Model Based on Studies in Medicago truncatula SE and Biotechnology References 2 Microspore Embryogenesis A. OLMEDILLA Introduction Discovery of the Production of Haploids by Anther Culture Strategies for the Induction of Microspore Embryogenesis Influence of Different Factors in Microspore Embryogenesis Cellular and Molecular Events Associated with Microspore Embryogenesis Conclusions References 3 Stress and Somaclonal Variation A.M. VÁZQUEZ and R. LINACERO Introduction Stress Responses in Plants Tissue Culture Imposes a Stress to the Cultivated In VitroCells Cultured Cells and Regenerated Plants Showed Variations Concluding Remarks References Part II Plant Processes and Its Regulation 4 Photosynthate PartitioningN.G. HALFORD Introduction Source and Sink Sugars as Signalling Molecules Key Metabolic Regulators Applications in Biotechnology Concluding Remarks References 5 Molecular Physiology of Seed Maturation and Seed Storage Protein BiosynthesisH. WEBER, N. SREENIVASULU, and W. WESCHKE Introduction Seed Maturation Sucrose as a Maturation Signal Synthesis and Deposition of Storage Proteins in Crop Seeds Storage Proteins in Cereals Metabolic Control of Seed Storage Protein Synthesis Outlook References 6 Fatty Acid Biosynthesis and Regulation in PlantsR. RAJASEKHARAN and V. NACHIAPPAN Introduction Fatty Acid Biosynthesis Fatty Acid Elongation Fatty Acid Desaturation Unusual Fatty Acids Assembly of Fatty Acids Conclusions References 7 Biosynthesis and Regulation of Carotenoids in Plants - Micronutrients, Vitamins and Health BenefitsC. I. CAZZONELLI, N. NISAR, D. HUSSAIN, M. CARMODY, and B.J. POGSON Introduction: Carotenoid Biosynthesis in Higher Plants Carotenoids and Plant Development Health Benefits of Carotenoid-Deri