Inicio > Matemáticas y ciencia > Biologia, ciencias de la vida > PCR per il rilevamento di E. Coli in pazienti affetti da diarrea
PCR per il rilevamento di E. Coli in pazienti affetti da diarrea

PCR per il rilevamento di E. Coli in pazienti affetti da diarrea

Mamoona Naz / Saher Shahid / Shaista Bashir

58,96 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786209202476
58,96 €
IVA incluido
Disponible

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Le malattie diarroiche batteriche rappresentano una delle cause significative di mortalità e morbilità umana nei paesi in via di sviluppo che non dispongono di standard sanitari pubblici adeguati in materia di igiene. L’Escherichia coli è riconosciuta come la principale causa di diarrea. Lo scopo del presente studio era quello di rilevare il gene bfp nei campioni di feci di pazienti affetti da diarrea mediante la reazione a catena della polimerasi, al fine di facilitarne la diagnosi. In questo studio, l’E. coli è stato rilevato nell’87,5% dei campioni di pazienti affetti da diarrea attraverso la produzione di colonie caratteristiche su piastre di agar MacConkey. Sulle piastre TSI inclinate, il 62,5% dei campioni di pazienti affetti da diarrea è stato identificato come E. coli. È stato riscontrato che una concentrazione di 3,5 mM ha prodotto i risultati migliori e che 0,5 µg di template è stata la concentrazione ottimale richiesta per l’amplificazione specifica del gene bfp. Il 20% dei ceppi puri di E. coli di pazienti affetti da diarrea ha mostrato risultati positivi per il gene bfp di E. coli enteropatogenico con il test PCR.

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