Microtubule Dynamics

1. How to Measure Microtubule Dynamics? Anne Straube 2. Purification of Tubulin from Porcine Brain Christopher Gell, Claire T. Friel, Barbara Borgonovo, David N. Drechsel, Anthony A. Hyman, and Jonathon Howard 3. Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe Douglas R Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, and Robert A. Cross 4. Generation of Differentially Polyglutamylated Microtubules Benjamin Lacroix and Carsten Janke 5. Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms Laurence Lafanechère and Didier Job 6. ab-Tubulin and Microtubule Binding Assays Jaime N. Campbell and Kevin C. Slep 7. Analysis of Microtubule Assembly Kinetics Using Turbidimetry Felicia Gaskin 8. Isolation of Centrosomes from Cultured Cells Simone Reber 9. Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds Miho Katsuki, Etsuko Muto, and Rob Cross 10. Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy Ivo A. Telley, Peter Bieling, and Thomas Surrey 11. Force Generation by Dynamic Microtubules In Vitro Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom 12. Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins Susanne Bechstedt, Michal Wieczorek, Michael Noujaim, and Gary J. Brouhard 13. Analyzing the ATP Hydrolysis Cycle of Microtubule Motors Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard 14. Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components Frédéric M. Coquelle, Sophie Blestel, Claire Heichette, Isabelle Arnal, Charles Kervrann, and Denis Chrétien 15. Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy Andreas Merdes and Jan De Mey 16. Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells Catarina P. Samora and Andrew D. McAinsh 17. Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures Xiaodong Zhu and Irina Kaverina 18. Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents Stéphane Honore and Diane Braguer 19. Laser Ablation of the Microtubule Cytoskeleton: Setting Up and Working with an Ablation System Nicola Maghelli and Iva Tolić-Nørrelykke 20. Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos Robin Antrobus and James G. Wakefield 21. Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques Ka Lou Yu, Nanda Keijzer, Casper C. Hoogenraad, and Anna Akhmanova