Microbial Metabolic Engineering

Table of ContentsPrefacePreamble1 Screening for Cellulases with Industrial Value and their use in Biomass ConversionJulia Jürgensen, Nele Ilmberger and Wolfgang R. Streit2 Reversal of NAD(P)H Cofactor Dependence by Protein EngineeringSabine Bastian and Francis H. Arnold3 Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution Samuel Million-Weaver, David L. Alexander, Jennifer M. Allen, and Manel Camps4 High Isoprenoid Flux Escherichia coli as a Host for Carotenoids ProductionWonchul Suh5 Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7 Nikhil U. Nair and Huimin Zhao6 Creation of New Metabolic Pathways or Improvement of Existing Metabolic Enzymes by in vivo Evolution in Escherichia coliIsabelle Meynial-Salles and Philippe Soucaille 7 Bioluminescent Reporter Genes for Promoter Discovery Tina K. Van Dyk8 Recombination-based DNA Assembly and Mutagenesis Methods for Metabolic Engineering Xiquan Liang, Lansha Peng, Billyana Tsvetanova, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen9 Ethanol-tolerant Gene Identification in Clostridium thermocellum using Pyro-resequencing for Metabolic EngineeringShihui Yang, Dawn M. Klingeman, and Steven D. Brown10 Use of Proteomic Tools in Microbial Engineering for Biofuel ProductionShaomin Mao, Kaizhi Jia, Yanping Zhang, Yin Li 11 Metabolic Engineering of Antibiotic-producing Actinomycetes using in vitro Transposon MutagenesisAndrew R. Reeves and J. Mark Weber12 Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide ProductionQiong Cheng, Kristin Ruebling-Jass, Jianzhong Zhang, Qi Chen and Kevin Croker13 Using Flux Balance Analysis to Guide Microbial Metabolic Engineering Kathleen A. Moran, Nathan C. Crook and Hal S. Alper14 Using an Advanced Microfermentor System for Strain Screening and Fermentation OptimizationDongming Xie15 Rapid Strain Evaluation using Dynamic DO-stat fed-batch Fermentation Under Scale-down Conditions Jun Sun16 Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanolgenic YeastsYing Zha, Ronald Slomp, Johan van Groenestijn and Peter Punt17 Engineering Whole-cell Biosensors with No Antibiotic Markers for Monitoring Aromatic Compounds in the EnvironmentAitor de las Heras and Víctor de Lorenzo18 Metabolic Engineering for Acetate Control in Large Scale FermentationYong Tao, Qiong Cheng and Alexander D, Kopatsis19 Minimization and Prevention of Phage Infections in BioprocessesMarcin Los Shaomin Mao, Kaizhi Jia, Yanping Zhang, Yin Li 11 Metabolic Engineering of Antibiotic-producing Actinomycetes using in vitro Transposon MutagenesisAndrew R. Reeves and J. Mark Weber12 Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide ProductionQiong Cheng, Kristin Ruebling-Jass, Jianzhong Zhang, Qi Chen and Kevin Croker13 Using Flux Balance Analysis to Guide Microbial Metabolic Engineering Kathleen A. Moran, Nathan C. Crook and Hal S. Alper14 Using an Advanced