Librería Samer Atenea
Librería Aciertas (Toledo)
Kálamo Books
Librería Perelló (Valencia)
Librería Elías (Asturias)
Donde los libros
Librería Kolima (Madrid)
Librería Proteo (Málaga)
The human calcium sensing receptor (hCaR) is aG-protein coupled receptor that responds to calciumions as its principal physiological agonist. L-aminoacids are allosteric activators of the cloned CaRexpressed in HEK293 cells but the physiologicalsignificance of this effect has been uncertain. Thisbook provides evidence that L-amino acids arephysiologically relevant activators of endogenousCaRs. Experiments reported in this book alsoinvestigated the location of amino acid binding siteof the CaR. Chimeric receptors constructed by domainshuffling between the human CaR and rat metabotropicglutamate receptor type-1 (mGlu-1) were used tolocalize the amino acid binding site of the CaR toits N-terminal, bilobed, Venus Fly Trap domain.Furthermore, the roles of putative amino acid bindingsite residues in the Venus Fly Trap domain wereinvestigated using conservative and semi-conservativemutations of the following residues: T145, S147,T151, S169, S170, S171, Y218 and E297. Finally, anovel activating mutation (E604K) in the cysteinerich domain of the hCaR in an Australian family withautosomal dominant hypocalcemia (ADH) was identified.
