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Keimungsgene von Bacillus thuringiensis subsp. israelensis

Keimungsgene von Bacillus thuringiensis subsp. israelensis

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786209273773
58,88 €
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Sobre IBD

Bacillus thuringiensis subsp. israelensis ist das wichtigste biologische Insektizid zur Bekämpfung von krankheitsübertragenden Mücken. Dieses Bakterium produziert während der Sporenbildung Toxine, die durch Lyse der Zellen im Mitteldarm zum Tod der Larven führen. Das Plasmid pBtoxis von B. thuringiensis subsp. israelensis kodiert alle mückenabtötenden Toxine und eine Reihe weiterer kodierender Sequenzen. Das Plasmid trägt auch potenzielle Keimungsgene, die in einem einzigen ger-Operon organisiert sind. Ein Vergleich der Keimungsreaktionen von Sporen aus Stämmen mit und ohne pBtoxis ergab, dass dieses Plasmid die Aktivierung der Sporen unter alkalischen Bedingungen, jedoch nicht nach einer Wärmebehandlung fördern kann. Die Einführung des ger-Operons auf einem rekombinanten Plasmid in den plasmidlosen Stamm etablierte dieses Operon als das erste mit einer identifizierten Rolle bei der alkalischen Aktivierung.

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