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Impacto da criopreservação de células mononucleares

Impacto da criopreservação de células mononucleares

Nour Louati

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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786208698355
58,96 €
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Sobre IBD

Desenvolver a técnica de separação dos CMNSPs em Ficoll e de descongelação e congelação dos mesmos, e avaliar o impacto da congelação a -80°C, a curto e longo prazo, nos dados de imunofenotipagem dos linfócitos.Dez casos de SLPC encaminhados para a unidade CMF: 8 casos de SLPC-B (4 casos de CLL e 4 casos de SLPC-B que não CLL) e 1 caso de SLPC-T (síndroma de Sézary) e 1 caso de SLPC com células NK.A comparação da viabilidade celular antes da congelação (D1) e após a congelação a -80°C (D5 e D30) revelou uma diminuição significativa da viabilidade celular após a congelação a curto prazo (D5) e a longo prazo (D30) (p

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