Inicio > Matemáticas y ciencia > Biologia, ciencias de la vida > Hodowla komórek BHK-21 w dostosowanej pożywce zawiesinowej bez surowicy
Hodowla komórek BHK-21 w dostosowanej pożywce zawiesinowej bez surowicy

Hodowla komórek BHK-21 w dostosowanej pożywce zawiesinowej bez surowicy

Jaci Leme

48,57 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Biologia, ciencias de la vida
ISBN:
9786208659509
48,57 €
IVA incluido
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Sobre IBD

Komórki ssaków są najczęściej wykorzystywanymi gospodarzami do produkcji białek biofarmaceutycznych i szczepionek wirusowych. Jakość jest kluczowym elementem w tworzeniu wydajnego procesu biokonwersji. W tym badaniu wykorzystaliśmy linię komórkową BHK-21C13 (Baby Hamster Kidney) przystosowaną do hodowli w zawiesinie. Użycie płodowej surowicy bydlęcej (FBS) było tradycyjnie stosowane i jest uważane za uniwersalny suplement, ponieważ umożliwia wzrost w różnych liniach komórkowych ssaków; jednak stosowanie FBS wiąże się z ryzykiem zakażenia prionami, zmiennością między partiami i wzrostem kosztów dalszego przetwarzania. Celem tego badania było porównanie hodowli komórek BHK-21 C13 między dwoma podłożami uzupełnionymi SFB i bez SFB, poprzez badania kinetyczne dla statycznej i mieszanej hodowli zawiesinowej przy użyciu odpowiednio kolby T, kolby obrotowej i bioreaktora.

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