Glycobiology Protocols

The Application of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues Ningguo Gao Partial Purification of Mannosylphosphorylundecaprenol Synthase from Micrococcus luteus: A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products Jeffrey S. Rush and Charles J. Waechter Assay of Dolichyl-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix John S. Schutzbach O-Mannosylation in Mammalian Cells Tamao Endo and Hiroshi Manya Methods for Analysis of Unusual Forms of O-glycosylation Aleksandra Nita-Lazar and Robert S. Haltiwanger Structural Analysis of Naturally Occurring Sialic Acids Johannis P. Kamerling and Gerrit J. Gerwig Non-Radioactive Trans-Sialidase Screening Assay Silke Schrader and Roland Schauer Structural Determination of O-Glycans by Tandem Mass Spectrometry Catherine Robbe, Jean-Claude Michalski, and Calliope Capon Detailed Structural Analysis of N-glycans Released from Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined with Exoglycosidase Array Digestions Louise Royle, Catherine M. Radcliffe, Raymond A. Dwek, and Pauline M. Rudd Molecular Modelling of Glycosyltransferases Anne Imberty, Michaela Wimmerova, Jaroslav Koca, and Christelle Breton b-Galactoside a2,6 Sialyltransferase (ST6Gal.I) and the Sialyl a2,6-galactosyl-linkage in Tissues and Cell Lines Fabio Dall’Olio, Nadia Malagolini, and Mariella Chiricolo Visualizing Intracellular Distribution and Activity of Core 2 b(1,6)N-acetylglucosaminyltransferase-I (C2GnT-I) in Living Cells Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari Gene Expression Analysis of Glycosylation-Related Genes by Real-Time PCR Juan J. Garcia-Vallejo, Sonja I. Gringhuis, Willem van Dijk, and Irma van Die Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells Inka Brockhausen, Xiaojing Yang, and Mark Harrison Micromethods for the Characterization of Lipid A-Core and O Antigen Lipopolysaccharide Cristina L. Marolda, Piya Lahiry, Enrique Vines, Soledad Saldias, and Miguel A. Valvano Assays for a Galactosyltransferase Involved in the Assembly of the O7 Antigen Repeat Unit of Escherichia coli Inka Brockhausen, Walter A. Szarek, John G. Riley, and Jason Vlahakis High Through-Put Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proximity Assay Utilizing Binding to Cationic Dyes Karen J. Rees-Milton and Tassos P. Anastassiades Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect ELISA Pablo Argueso and Ilene K. Gipson Molecular Dissection of the Mouse Zona Pellucida: An Electron Microscopic Perspective Utilizing High Resolution Colloidal-Gold Labeling Methods Frederick W.K. Kan Soluble Adamantly GSL Analogues as Probes of GSL Function Clifford Lingwood, Skanda Sadacharan, Maan Abul-Milh, Murugespillai Mylvaganum, and Marcus Peter Diagnosis of Krabbe Disease by Use of a Natural Substrate John W. Callahan and Marie-Anne Skomorowski In Vitro Assays of the Functions of Calnexin and Calreticulin, Lectin-Chaperones of the Endoplasmic Reticulum Breanna S. Ireland, Monika Niggemann, and David B.Williams Quantitative Measurement of Selectin-Ligand Interactions: Assays to Identify a Sweet Pill in a Library of Carbohydrates Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta Sialic Acid Binding Specificities of Siglecs Nadine Bock and Soerge Kelm