DNA Repair Protocols

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating Malgorzata Z. Zdzienicka Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities William W. Au and Salama A. Salama Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation Shruti Nagar, James J. Corcoran, and William F. Morgan Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication George Iliakis, Ya Wang, and Hong Yan Wang Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage Xuan Huang and Zbigniew Darzynkiewicz Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication) Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi In Vitro Rejoining of Double-Strand Breaks in Genomic DNA George Iliakis and Nge Cheong Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan Plasmid-Based Assays for DNA End-Joining In Vitro George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants Rodney S. Nairn and Gerald M. Adair Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr Quantitative PCR-Based Measurement of Nuclear and MitochondrialDNA Damage and Repair in Mammalian Cells Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR Gerd P. Pfeifer Immunochemical Detection of UV-Induced DNA Damage and Repair Marcus S. Cooke and Alistair Robson A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts Shirley McCready Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay David L. Mitchell DNA Damage Quantitation by Alkaline Gel Electrophoresis Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair Günter Speit and Andreas Hartmann Fast Micromethod DNA Single-Strand-Break Assay Heinz C. Schröder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Müller 32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA Vaughn Smider, Byung Joon Hwang, and Gilbert Chu Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision Huixian Wang and John B. Hays Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites Walter A. Deutsch and Vijay Hegde Base Excision Repair in Mammalian Cells Yoshihiro Matsumoto In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti Biochemical Assays for the Characterization of DNA Helicases Robert M. Brosh, Jr. and Sudha Sharma Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell