Dendritic Cell Protocols

Part I: Reviews1. Review of Human DC subtypesXinsheng Ju, Georgina Clark, and Derek N.J. Hart2. Review of Murine Dendritic Cells: Types, Location, and DevelopmentTewfik Miloud, Günter J. Hämmerling, and Natalio GarbiPart II: Human DC Protocols3. Isolation of Human Blood DC SubtypesAndrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, and Kristen J. Radford4. Isolation of Immature Primary Langerhans Cells from Human Epidermal SkinMarein A.W.P. de Jong, Lot de Witte, and Teunis B.H. Geijtenbeek5. Isolation and in vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, and Bianca Blom6. In vivo Modulation of Gene Expression by Lentiviral Transduction in ’Human Immune System’ Rag2-/-gc-/- Mice Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan Pouw, Kees Weijer, Hergen Spits, Bianca Blom, and Nicolas Legrand7. Generation of Stable Th1/CTL-, Th2- and Th17-inducing Human Dendritic Cells Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and Esther de Jong8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in TransplantationHeth R. Turnquist, Ryan Fischer, and Angus W. Thomson9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell VaccinesMichael Erdmann and Beatrice Schuler-ThurnerPart III: Murine Dendritic Cell Methods10. Generation of Mouse CD8+, CD8-, and Plasmacytoid Dendritic Cells Equivalents in vitro Using flt3 LigandShalin H. Naik, Meredith O’Keeffe, Anna Proietto, Hubertus Hochrein, KenShortman, and Li Wu11. Generation of Large Numbers of pro-DCs and pre-DCs in vitroShalin H. Naik12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSFMeredith O’Keeffe, Ben Fancke, and Hubertus Hochrein13. Isolation of Common Dendritic Cell Progenitor (CDP) from Mouse Bone MarrowNobuyuki Onai, Markus G. Manz, and Michael A. Schmid14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow CytometryDavid Vremec15. Isolation of Cutaneous Dendritic Cells Julie Helft and Miriam Merad16. Isolation of Skin Dendritic Cells from Mouse and ManPatrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, and Susanne Ebner17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, and Patrick G. Holt18. Isolation of Mouse Thymic Dendritic Cell PrecursorsLi Wu and Angela D’Amico19. Isolation of Rat Intestinal Lymph DCSimon Milling and Gordon MacPherson20. Mouse Models of Viral Infection: Influenza Infection in the LungAdele M. Mount and Gabrielle T. Belz21. DCs in Mouse Models of Intracellular Bacterial InfectionMichael Neuenhahn, Matthias Schiemann, and Dirk H. Busch22. Studying the Function of Dendritic Cells in Mouse Models of AsthmaPhilippe Pouliot, Monique A. Willart, Hamida Hammad, and Bart N. Lambrecht23. Direct ex vivo Activation of T cells for Analysis of Dendritic Cell Antigen PresentationGabrielle T. Belz24. Methods to Study Pulmonary Dendritic Cell Migration Claudia Jakubzick and