Cytokine Protocols

1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender DiscoveryAlfons Billiau2 The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA LevelsMaria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes3 Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVIDTomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki4 Quantitative Analysis of miRNA Expression in Epithelial Cells and TissuesMarkus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil 5 Evaluating Posttranscriptional Regulation of Cytokine GenesBernd Rattenbacher and Paul R. Bohjanen6 Cloning of Cytokine 3’ Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay Latifa Al-Haj and Khalid S.A. Khabar7 Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivoCharudharshini Srinivasan, Dan Peer, and Motomu Shimaoka8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth FactorPaulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz9 Control of the Interferon Response in RNAi ExperimentsJana Nejepinska, Matyas Flemr, and Petr Svoboda 10. shRNA-induced Interferon-Stimulated Gene AnalysisNúria Morral and Scott R. Witting11 Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta CellsFabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik12 Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding ProteinsDaniela Novick and Menachem Rubinstein13 In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic CellsWilliam C. Adams and Karin Loré 14 A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH CellsLennart Zabeau, José Van der Heyden, and Jan Tavernier