Chemical Genomics

Part I. Reviews Introduction to Chemical Genomics Paul R. Caron Chemistry for Chemical Genomics Lutz Weber Computer-Aided Design of Small Molecules for Chemical Genomics Philip M. Dean Part II. Protocols Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe The Role and Application of In Silico Docking in Chemical Genomics Research Aldo Jongejan, Chris de Graaf, Nico P. E. Vermeulen, Rob Leurs, and Iwan J. P. de Esch Synthesis of Complex Carbohydrates and Glyconjugates: Enzymatic Synthesis of Globotetraose Using b-1,3-N-Acetylgalactosaminyltransferase LgtD From Haemophilus infuenzae Strain Rd Kang Ryu, Steven Lin, Jun Shao, Jing Song, Min Chen, Wei Wang, Hanfen Li, Wen Yi, and Peng George Wang High-Throughput Cloning for Proteomics Research Sharon A. Doyle Screening for the Expression of Soluble Recombinant Protein in Escherichia coli Sharon A. Doyle High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli Michael B. Murphy and Sharon A. Doyle The Wheat Germ Cell-Free Expression System: Methods for High-Throughput Materialization of Genetic Information Tatsuya Sawasaki, Mudeppa D. Gouda, Takayasu Kawasaki, Takafumi Tsuboi, Yuzuru Tozawa, Kazuyuki Takai, and Yaeta Endo Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System Yaeta Endo and Tatsuya Sawasaki Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System Toshiyuki Kohno Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening Jon Hoyt and Randall W. King Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis Jonathan M. Blackburn and Darren J. Hart Peptideand Small-Molecule Microarrays Jan Marik and Kit S. Lam Peptide Mass Fingerprinting: Protein Identification Using MALDI-TOF Mass Spectrometry Judith Webster and David Oxley A Practical Protocol for Carbohydrate Microarrays Ruobing Wang, Shaoyi Liu, Dhaval Shah, and Denong Wang Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors Vladimir Khazak, Erica A. Golemis, and Lutz Weber Index