Cell Migration

Part I. Introduction Cell Migration: An Overview Donna J. Webb, Huaye Zhang, and Alan F. Horwitz Part II. Basic Cell Migration and Related Assays Boyden Chamber Assay Hong-Chen Chen Wound-Healing Assay Luis G. Rodriguez, Xiaoyang Wu, and Jun-Lin Guan Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy Claire M. Wells and Anne J. Ridley Cell-Adhesion Assays Dennis F. Kucik and Chuanyue Wu Cell-Spreading Assays Allison L. Berrier and Susan E. LaFlamme Cell-Scatter Assay Hong-Chen Chen Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices Edna Cukierman Part III. Analysis of Cell Migration in Specialized Cell Systems Tumor Cell Invasion Assays Leslie M. Shaw Analysis of Endothelial Cell Migration Under Flow Song Li Angiogenesis Assays in the Chick CAM Chris Storgard, David Mikolon, and Dwayne G. Stupack Investigations of Neuronal Migration in the Central Nervous System Michael E. Ward and Yi Rao Part IV. Analysis of Cell Migration in Model Organisms Analysis of Cell Migration in Caenorhabditis elegans M. Afaq Shakir and Erik A. Lundquist Analysis of Cell Migration Using Drosophila as a Model System Jocelyn A. McDonald and Denise J. Montell Imaging Cell Movement During Dorsal Closure in Drosophila Embryos William Wood and Antonio Jacinto Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo Diane S. Sepich and Lilianna Solnica-Krezel The Xenopus Embryo as a Model System for Studies of Cell Migration Douglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique Alfandari Neural Crest Migration Methods in the Chicken Embryo Maria Elena de Bellard and Marianne Bronner-Fraser In Vivo and In Vitro Models of Mammalian Angiogenesis Mien V. Hoang and Donald R. Senger Part V. Biochemical and Novel Approaches in Cell Migration Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. Schaller Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches Frauke Drees, Amy Reilein, and W. James Nelson Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells Christoph Ballestrem and Benjamin Geiger Use of Multiphoton Imaging for Studying Cell Migration in the Mouse Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu. Nikitin Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin Guan Index