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Wkład biologii molekularnej w diagnostykę pneumocystis

Wkład biologii molekularnej w diagnostykę pneumocystis

Boutheina Jemli / Eya SESSI / Latifa Mtibaa

85,21 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2025
Materia
Medicina: cuestiones generales
ISBN:
9786208786892
85,21 €
IVA incluido
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Pneumocystis jest oportunistyczną infekcją, która dotyka głównie niedojrzałych pacjentów. Wprowadzenie biologii molekularnej do diagnostyki pneumocystis poprawiło czułość wykrywania patogenu. Celem naszego badania jest wykazanie udziału PCR w czasie rzeczywistym w porównaniu z technikami barwienia. Nasze badanie ma charakter retrospektywny (styczeń 2022-sierpień 2023) i obejmuje 6 przypadków pneumocystis. W 4 przypadkach (67%) wykryto współzakażenia obejmujące Pneumocystis jirovecii w połączeniu z HIV, CMV i SARS-CoV-2. Współzakażenie Mycobacterium tuberculosis zaobserwowano w 1 przypadku (17%). Konwencjonalne barwienie MGG i Musto-modyfikowanym Gomori Grocott było ujemne we wszystkich przypadkach. Jednak analiza PCR w czasie rzeczywistym wykazała pozytywne wyniki dla wszystkich próbek, wykazując 100% czułość. Poziomy obciążenia pasożytami wahały się od 10 do106 kopii/ml. Ilościowy PCR jest wysoce czułą techniką, z mniejszym ryzykiem zanieczyszczenia i pomaga klinicystom odróżnić kolonizację od infekcji.

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