Bernardo D. C. Martins / Bruno B. J. Torres / Eliane G. de Melo
Librería Samer Atenea
Kálamo Books
Librería Elías (Asturias)
Librería Kolima (Madrid)
Librería Proteo (Málaga)
The effect of mesenchymal stem cells (MSCs) and dantrolene (DAN) was evaluated in rats with experimental acute spinal cord injury. Sixty three-month-old male Wistar rats were used, divided into five groups: MSC; DAN + MSC; DAN; positive control (PC) (trauma and placebo); negative control (NC) (no trauma). A T12 laminectomy was performed on all animals, followed by compressive trauma, with the exception of the NC group, using a stereotaxic apparatus with a 70g weight for five minutes. One hour later, the DAN + CTM and DAN groups received a single intraperitoneal dose of 10 mg/kg of DAN, and after seven days, the CTM and DAN + CTM groups received 1 million cells intravenously. Behavioural tests were performed to assess functional recovery using the BBB scale and the adapted descriptive scale method over a 30-day period. Following euthanasia, samples were collected from various organs and the spinal cord for histological evaluation in H.E. To assess spinal cord integrity, immunohistochemistry was performed using anti-NeuN. Real-time RT-PCR was also performed to assess the gene expression of neurotrophic and apoptotic factors.
