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Haplodiploidisierung bei Hartweizen durch Androgenese

Haplodiploidisierung bei Hartweizen durch Androgenese

Laila RHAZI / Mohamed Ben Bammou / Mouhssine RHAZI

61,69 €
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Editorial:
KS OmniScriptum Publishing
Año de edición:
2024
Materia
Biologia, ciencias de la vida
ISBN:
9786207045082
61,69 €
IVA incluido
Disponible

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Haploiden greifen vorteilhaft in die Züchtung von Getreidesorten ein.Die Methode der Haplodiploidisierung: In-vitro-Androgenese wurde bei vier Hartweizensorten (Marzak, Massa, Oum rabia und Tomouh) angewandt.Neben dem Effekt des Genotyps wurden die verschiedenen Ergebnisse (Kallus, Embryonen und Pflanzen) in Abhängigkeit vom Spendertalus, der Kältevorbehandlung und dem Kulturmedium (C17 und Olsen) analysiert. Der Spendertalus zeigt einen signifikanten Effekt auf die Induktion von Embryonen und Kallus, wobei der Masterstrang besser abschneidet als der erste Talus.Es zeigte sich ein nicht signifikanter Effekt der Kältevorbehandlung auf die Embryoinduktion und die Kallusproduktion, aber auch auf die Qualität der Regenerate. Es wurde auch festgestellt, dass ein einziger auf Olsen-Medium erzeugter Embryo eine chlorophyllhaltige Pflanze hervorbrachte, während die auf C17-Medium erzeugten Embryonen keine Pflanzen hervorbrachten; Albinismus war nicht vorhanden. Es wird betont, dass der Genotyp-Faktor bei der Embryoinduktion und Regeneration sehr wichtig ist. Die Ergebnisse zeigen, dass Hartweizen ein widerspenstiger Genotyp bleibt, insbesondere bei der Regeneration von Sämlingen.

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